Response Surface Methodology Optimization of Ultrasonic-Assisted Extraction of Acer Truncatum Leaves for Maximal Phenolic Yield and Antioxidant Activity.

نویسندگان

  • Lingguang Yang
  • Peipei Yin
  • Hang Fan
  • Qiang Xue
  • Ke Li
  • Xiang Li
  • Liwei Sun
  • Yujun Liu
چکیده

This study is the first to report the use of response surface methodology to improve phenolic yield and antioxidant activity of Acer truncatum leaves extracts (ATLs) obtained by ultrasonic-assisted extraction. The phenolic composition in ATLs extracted under the optimized conditions were characterized by UPLC-QTOF-MS/MS. Solvent and extraction time were selected based on preliminary experiments, and a four-factors-three-levels central composite design was conducted to optimize solvent concentration (X₁), material-to-liquid ratio (X₂), ultrasonic temperature (X₃) and power (X₄) for an optimal total phenol yield (Y₁) and DPPH• antioxidant activity (Y₂). The results showed that the optimal combination was ethanol:water (v:v) 66.21%, material-to-liquid ratio 1:15.31 g/mL, ultrasonic bath temperature 60 °C, power 267.30 W, and time 30 min with three extractions, giving a maximal total phenol yield of 7593.62 mg gallic acid equivalent/100 g d.w. and a maximal DPPH• antioxidant activity of 74,241.61 μmol Trolox equivalent/100 g d.w. Furthermore, 22 phenolics were first identified in ATL extract obtained under the optimized conditions, indicating that gallates, gallotannins, quercetin, myricetin and chlorogenic acid derivatives were the main phenolic components in ATL. What's more, a gallotannins pathway existing in ATL from gallic acid to penta-O-galloylglucoside was proposed. All these results provide practical information aiming at full utilization of phenolics in ATL, together with fundamental knowledge for further research.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Optimization of Ultrasonic-Assisted Extraction by Response Surface Methodology with Maximal Phenolic Yield and Antioxidant Activity from Acer truncatum Leaves

This study was designed for the first time to improve phenolic yield and antioxidant activity of ultrasonic-assisted extraction from Acer truncatum leaves (ATL) using response surface methodology, and phenolic composition in ATL extracted under the optimized condition were characterized by UPLC-QTOF-MS/MS. Solvent and extraction time were selected based on preliminary experiments, and a four-fa...

متن کامل

Optimization of Ultrasonic-Assisted Extraction of Natural Antioxidants from Elaegnus Angustifolia Using Response Surface Methodlogy

Restrictions on the use of synthetic antioxidant such as butylated  hydroxyanisole  (BHA) and butylated  hydroxytoluene (BHT) are being imposed  because of  their carcinogecity. In this investigation  optimization  of phenolic extraction  process  frome  methanolic (80%)  extract (v/v) of  elaegnus  angustifolia  through  Response  Surface Methodology  by fulin cio- calteu is  performrd  for op...

متن کامل

Optimization of Ultrasonic-Assisted Extraction of Natural Antioxidants from Elaegnus Angustifolia Using Response Surface Methodlogy

Restrictions on the use of synthetic antioxidant such as butylated  hydroxyanisole  (BHA) and butylated  hydroxytoluene (BHT) are being imposed  because of  their carcinogecity. In this investigation  optimization  of phenolic extraction  process  frome  methanolic (80%)  extract (v/v) of  elaegnus  angustifolia  through  Response  Surface Methodology  by fulin cio- calteu is  performrd  for op...

متن کامل

A Multi-Objective Optimization of Artichoke (Cynara Scolymus L.) Leaves Aqueous Extraction Dehydration Through a Novel Spray Drying Approach Using Response Surface Methodology

In this study, Cynara scolymus L. leaves aqueous extract powder was produced using a novel spray drying approach by incorporation of ongoing ultrasonic atomization and vacuumed drying chamber. A Response Surface Methodology (RSM)-based central composite face-centered design was employed for optimization of the operating conditions of the ultrasonic vacuum spray dryer. The independent variab...

متن کامل

Evaluation, prediction and optimization the ultrasound-assisted extraction method using response surface methodology: antioxidant and biological properties of Stachys parviflora L.

Objective(s):To optimize the extraction method using response surface methodology, extract the phenolic compounds, and identify the antioxidant and biological properties of Stachys parviflora L.  extracts. Materials and Methods: Maceration and ultrasound-assisted extraction (UAE) (4, 7, 10 min treatment time, 40, 70, 100 % high-intensity and 60, 80, 100 % (v v-1) methanol purity) were applied t...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Molecules

دوره 22 2  شماره 

صفحات  -

تاریخ انتشار 2017